Comprehensive developmental somatic proteome atlas of Haemonchus contortus underpinned by a chromosome-scale genome and deep tandem mass spectrometry

Abstract

Background: Haemonchus contortus is a highly pathogenic, blood-feeding nematode that causes widespread disease and significant economic loss in livestock worldwide. Previous proteomic studies were constrained by incomplete genomic resources and limited analytical sensitivity, impeding comprehensive profiling across life stages. Methods: In this study, we integrated advanced tandem mass spectrometry with a chromosome-scale genome assembly of the Haecon-5 strain to construct the most detailed somatic proteome of H. contortus to date. Results: We identified and quantified 7002 proteins across five key developmental stages/sexes—i.e. eggs, third-stage larvae (L3s), fourth-stage larvae (L4s), and..